Services

Cell pellets should be delivered in 1.5ml Eppendorf tubes, and ideally should be in the range of 5-10 Mio cells.

Cell extracts: please consult with PRI for buffer compatibility with our sample prep protocols. The sample concentration should be adjusted to a common concentration. A common format would be 50 µL RIPA extract of 2 µg/µL concentration.

‘Ready to inject samples’ should be provided in Thermo Scientific WebSeal Well plates (60180-P217B) closed with a rubber sealer. The sample volume should be a maximum of 50 µL and allow the extraction of 2.2 µL for concentration measurement. The sample concentration should be ideally adjusted to 0.1 µg/µL.

Samples for high-pH fractionation should be provided at a maximum concentration of 2 ug/µL. The maximum injection volume for the fractionator is 20 µL. If the sample amount is not limiting, we recommend fractionating 10 - 20 µg.  Samples for fractionation need to be cleaned previously by C18 or SDB-RPS.

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Please include all necessary sample information required for statistical analysis such as sample grouping as well as the required comparisons (e.g., ‘ko’ vs ‘wt’). For any additional information on the experiment please use the comment section.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

• ‘Analytical Sample ID’ is mandatory. If possible, please use alphanumeric entries with a consecutive number at the end (e.g., S1, S2, S3, …)
• If statistical analysis is requested, please also fill in the ‘Grouping’ column. Groupings should reflect the replicate groups used for statistical analysis (e.g., ‘ko’, ‘wt’).
• All other columns are optional in most cases with the following exceptions:
• ‘Biological Sample ID’ is optional and can be used for any additional information or internal sample ids.
• For samples provided in 96-well plates, please define Plate Id, Plate Position, Volume, and – if applicable – Concentration. Only registered plate Ids can be selected, and you can obtain a list of valid plate IDs together with barcoded label stickers from PRI.

Whenever possible, samples should be provided in barcoded 0.75 mL Micronic tubes. If samples have been already aliquoted, any other tube type is fine as well.

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Please include all necessary sample information required for statistical analysis such as sample grouping as well as the required comparisons (e.g., ‘treatment’ vs ‘control’). For any additional information on the experiment please use the comment section.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

• ‘Analytical Sample ID’ is mandatory. It can either represent the sample id (key cath) of the biobank, or an experiment specific id (S1, S2,…. Sx).
• Subject ID’ should contain the pseudonymized subject identifier.
• Biological Sample ID’ is optional and can be used for any additional information (e.g. timepoint 1 and 2)
• ‘Tube label’ needs to be filled in for barcoded tubes and represent the barcode id
• For samples provided in 96-well plates, please define Plate Id, Plate Position, and Only registered plate Ids can be selected, and you can obtain a list of valid plate IDs together with barcoded label stickers from PRI.

Samples can be submitted in tubes. For larger requests, we ask users to transfer the samples directly into some special plates, which ideally is done at our facility, so it can be done right before processing.

• ‘Analytical Sample ID’ is mandatory. It can either represent the sample id (key cath) of the biobank, or an experiment specific id (S1, S2,…. Sx).
• Subject ID’ should contain the pseudonymized subject identifier.
• Biological Sample ID’ is optional and can be used for any additional information (e.g. timepoint 1 and 2)

Cell pellets should be delivered in 1.5ml Eppendorf tubes, and ideally should be in the range of 5-10 Mio cells.

Cell extracts: please consult with PRI for buffer compatibility with our sample prep protocols. The sample concentration should be adjusted to a common concentration. A common format would be 50 µL RIPA extract of 2 µg/µL concentration.

‘Ready to inject samples’ should be provided in Thermo Scientific WebSeal Well plates (60180-P217B) closed with a rubber sealer. The sample volume should be a maximum of 50 µL and allow the extraction of 2.2 µL for concentration measurement. The sample concentration should be ideally adjusted to 0.1 µg/µL.

Samples for high-pH fractionation should be provided at a maximum concentration of 2 ug/µL. The maximum injection volume for the fractionator is 20 µL. If the sample amount is not limiting, we recommend fractionating 10 - 20 µg.  Samples for fractionation need to be cleaned previously by C18 or SDB-RPS.

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Please include all necessary sample information required for statistical analysis such as sample grouping as well as the required comparisons (e.g., ‘induction’ vs ‘control’). For any additional information on the experiment please use the comment section.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

• ‘Analytical Sample ID’ is mandatory. If possible, please use alphanumeric entries with a consecutive number at the end (e.g., S1, S2, S3, …)
• If statistical analysis is requested, please also fill in the ‘Grouping’ column. Groupings should reflect the replicate groups used for statistical analysis (e.g., ‘ko’, ‘wt’).
• All other columns are optional in most cases with the following exceptions:
• ‘Biological Sample ID’ is optional can be used for any additional information or internal sample ids.

For samples provided in 96-well plates, please define Plate Id, Plate Position, Volume, and – if applicable – Concentration. Only registered plate Ids can be selected, and you can obtain a list of valid plate IDs together with barcoded label stickers from PRI.

Samples are delivered as washed beads (magnetic or agarose). Following the regular washes of the enrichment experiment, beads need to be washed twice with detergent-free buffer (e.g., TBS or PBS). Please change the tube to a fresh 1.5 mL Eppendorf tube with the last wash. Remove the last wash buffer before freezing the beads or bringing them to the PRI on ice. 

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Please include all necessary sample information required for statistical analysis such as sample grouping as well as the required comparisons (e.g., ‘bait’ vs ‘control’). For any additional information on the experiment please use the comment section.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

• ‘Analytical Sample ID’ is mandatory. If possible, please use alphanumeric entries with a consecutive number at the end (e.g., S1, S2, S3, …)
• If statistical analysis is requested, please also fill in the ‘Grouping’ column. Groupings should reflect the replicate groups used for statistical analysis (e.g., ‘bait’, ‘control’).
• All other columns are optional in most cases with the following exceptions:
• ‘Biological Sample ID’ is optional and can be used for any additional information or internal sample ids.

Gel Bands should be provided in barcoded Micronic 0.75 mL hybrid tubes (can be obtained at PRI). When cutting the band, please remove as much of the surrounding gel as possible and chop up the band in cubes of about 2 mm edge length.

Please also acquire a picture of the gel before cutting the gel and upload the picture with marked bands into the comment section of the request form.

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

You can generate new samples with the ‘+ Sample’ button, or via down uploading a filled in template file. If you wish to use the template file, we recommend following order

• Generate first sample with the ‘+ Sample’ button
• Fill in the Analytical sample ID field for the first sample (e.g., ‘S1)
• Press ‘Export for Import’ button and download the table
• Open the table in Excel and add all additional samples and info.
• Import the table with the ‘Import’ button

Note the standard sorting of bookitlab is starting with the highest internal bookitlab ID, so the last sample in your list will be shown in the first row after this process. You can sort the samples in forward order by clicking on the ID column header.

The following columns need to be filled in:

• ‘Analytical Sample ID’ is mandatory. Please use alphanumeric entries with a consecutive number at the end (e.g., S1, S2, S3, …)
• ‘Tube label’ is mandatory and refers to the barcode indicated on the tube. Please enter the number with an underscore in front (e.g. ‘_4055436968’)
• Sample type should contain the term ‘gel band’
• Taxa ID should contain the Uniprot taxa id of the organism
• ‘Biological Sample ID’ is optional and can be used for any additional information or internal sample ids (e.g., ‘Band1’).
• All other columns are usually not relevant for this service type

Example for a filled request table to import:

Samples are submitted in solution. Please indicate the exact buffer composition and discuss about potential compatibility issues beforehand.

In the Service Details screen, please fill in all mandatory fields. The section ‘Add-on Charges’ is not relevant and can be ignored.

The ‘number of samples’ must match the number of samples delivered to PRI, and the number of rows in the 'Request Samples' table.

Once all metadata is entered, click on the ‘Request Samples’ link and enter sample information in the sample table.

The following columns need to be filled in:

• ‘Analytical Sample ID’ is mandatory. Please use alphanumeric entries with a consecutive number at the end (e.g., S1, S2, S3, …)
• Taxa ID should contain the Uniprot taxa id of the organism
• ‘Biological Sample ID’ is optional and can be used for any additional information or internal sample ids (e.g., ‘sample1’).
• All other columns are usually not relevant for this service type